Example: HER2, histone, P28482, P19174-2

Protein CLCB_HUMAN (P09497)

Canonical isoform: P09497-1 [UniProt]

Other possible isoforms:
P09497-2

HUGO gene symbols:

Protein name: Clathrin light chain B

Associated HotPTM clusters:

PTM sites

(Only the canonical isoform P09497-1 is shown)

Position Residue Category PTM Type Evidence (Manual/Automatic assertion)
11 S Phosphorylation Phosphoserine
Manual assertion inferred from sequence similarity [ECO:0000250]

UniProtKB: P04975

13 S Phosphorylation Phosphoserine
Manual assertion inferred from sequence similarity [ECO:0000250]

UniProtKB: P04975

87 Y Phosphorylation Phosphotyrosine
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
  • Curation set 4719 (2008)
  • Curation set 4457 (2008)
105 K Acetylation N6-acetyllysine
Mass spectrometry data [ECO:0007829]

From CPTAC3 GBM discovery

Proteogenomic and metabolomic characterization of human glioblastoma
Wang Liang-Bo, Karpova Alla, Gritsenko Marina A. et al.
Cancer Cell 39:509-528.e20 (2021). [PubMed] [Europe PMC]

122 K Ubiquitination Ubiquitination-Lys-Gly
Peer reviewed articles curated by PhosphoSitePlus®.

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Akimov Vyacheslav, Barrio-Hernandez Inigo, Hansen Sten V. F. et al.
Nature Structural & Molecular Biology 25:631-640 (2018). [PubMed] [Europe PMC]

134 K Ubiquitination Ubiquitination-Lys-Gly
Peer reviewed articles curated by PhosphoSitePlus®.

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Akimov Vyacheslav, Barrio-Hernandez Inigo, Hansen Sten V. F. et al.
Nature Structural & Molecular Biology 25:631-640 (2018). [PubMed] [Europe PMC]

149 K Ubiquitination Ubiquitination-Lys-Gly
Mass spectrometry data [ECO:0007829]

From CPTAC3 LSCC discovery

Unpublished data
149 K Acetylation N6-acetyllysine
Mass spectrometry data [ECO:0007829]

From CPTAC2 BRCA prospective

Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
Krug Karsten, Jaehnig Eric J., Satpathy Shankha et al.
Cell 183:1436-1456.e31 (2020). [PubMed] [Europe PMC]

From CPTAC3 UCEC discovery

Proteogenomic Characterization of Endometrial Carcinoma
Dou Yongchao, Kawaler Emily A., Cui Zhou Daniel et al.
Cell 180:729-748.e26 (2020). [PubMed] [Europe PMC]

184 K Ubiquitination Ubiquitination-Lys-Gly
Mass spectrometry data [ECO:0007829]

From CPTAC3 LSCC discovery

Unpublished data

Peer reviewed articles curated by PhosphoSitePlus®.

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Akimov Vyacheslav, Barrio-Hernandez Inigo, Hansen Sten V. F. et al.
Nature Structural & Molecular Biology 25:631-640 (2018). [PubMed] [Europe PMC]

187 T Phosphorylation Phosphothreonine
Mass spectrometry data [ECO:0007829]

From CPTAC3 GBM discovery

Proteogenomic and metabolomic characterization of human glioblastoma
Wang Liang-Bo, Karpova Alla, Gritsenko Marina A. et al.
Cancer Cell 39:509-528.e20 (2021). [PubMed] [Europe PMC]

From CPTAC3 HNSCC discovery

Proteogenomic insights into the biology and treatment of HPV-negative head and neck squamous cell carcinoma
Huang Chen, Chen Lijun, Savage Sara R. et al.
Cancer Cell 39:361-379.e16 (2021). [PubMed] [Europe PMC]

From CPTAC2 BRCA prospective

Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
Krug Karsten, Jaehnig Eric J., Satpathy Shankha et al.
Cell 183:1436-1456.e31 (2020). [PubMed] [Europe PMC]

From CPTAC3 LUAD discovery

Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
Gillette Michael A., Satpathy Shankha, Cao Song et al.
Cell 182:200-225.e35 (2020). [PubMed] [Europe PMC]

From CPTAC3 CCRCC discovery

Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma
Clark David J., Dhanasekaran Saravana M., Petralia Francesca et al.
Cell 179:964-983.e31 (2019). [PubMed] [Europe PMC]

From CPTAC3 LSCC discovery

Unpublished data

Peer reviewed articles curated by PhosphoSitePlus®.

Proteogenomics connects somatic mutations to signalling in breast cancer
Mertins Philipp, NCI CPTAC, Mani D. R. et al.
Nature 534:55-62 (2016). [PubMed] [Europe PMC]

Toward a comprehensive characterization of a human cancer cell phosphoproteome.
Zhou H., Di Palma S., Preisinger C. et al.
J. Proteome Res. 12:260-271 (2013). [PubMed] [Europe PMC]

Manual assertion inferred from algorithms based on publications or databases entries, e.g., large-scale proteomics data and 3D structure [ECO:0007744]

Toward a comprehensive characterization of a human cancer cell phosphoproteome.
Zhou H., Di Palma S., Preisinger C. et al.
J. Proteome Res. 12:260-271 (2013). [PubMed] [Europe PMC]

190 T Phosphorylation Phosphothreonine
Peer reviewed articles curated by PhosphoSitePlus®.

Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics
Tsai Chia-Feng, Wang Yi-Ting, Yen Hsin-Yung et al.
Nature Communications 6:None (2015). [PubMed] [Europe PMC]

194 K Ubiquitination Ubiquitination-Lys-Gly
Peer reviewed articles curated by PhosphoSitePlus®.

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Akimov Vyacheslav, Barrio-Hernandez Inigo, Hansen Sten V. F. et al.
Nature Structural & Molecular Biology 25:631-640 (2018). [PubMed] [Europe PMC]

194 K Methylation N6-methyllysine
Peer reviewed articles curated by PhosphoSitePlus®.

Quantitative Profiling of the Activity of Protein Lysine Methyltransferase SMYD2 Using SILAC-Based Proteomics
Olsen Jonathan B., Cao Xing-Jun, Han Bomie et al.
Molecular & Cellular Proteomics 15:892-905 (2016). [PubMed] [Europe PMC]

194 K Acetylation N6-acetyllysine
Mass spectrometry data [ECO:0007829]

From CPTAC3 GBM discovery

Proteogenomic and metabolomic characterization of human glioblastoma
Wang Liang-Bo, Karpova Alla, Gritsenko Marina A. et al.
Cancer Cell 39:509-528.e20 (2021). [PubMed] [Europe PMC]

From CPTAC2 BRCA prospective

Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
Krug Karsten, Jaehnig Eric J., Satpathy Shankha et al.
Cell 183:1436-1456.e31 (2020). [PubMed] [Europe PMC]

204 K Ubiquitination Ubiquitination-Lys-Gly
Mass spectrometry data [ECO:0007829]

From CPTAC3 LSCC discovery

Unpublished data

Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
  • Curation set 8643 (2009)
  • Curation set 8645 (2009)
  • Curation set 8641 (2009)
  • Curation set 8635 (2009)
  • Curation set 8636 (2009)
  • Curation set 8247 (2010)
Peer reviewed articles curated by PhosphoSitePlus®.

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Akimov Vyacheslav, Barrio-Hernandez Inigo, Hansen Sten V. F. et al.
Nature Structural & Molecular Biology 25:631-640 (2018). [PubMed] [Europe PMC]

Multiomic Analysis of the UV-Induced DNA Damage Response
Boeing Stefan, Williamson Laura, Encheva Vesela et al.
Cell Reports 15:1597-1610 (2016). [PubMed] [Europe PMC]

Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line
Wu Quan, Cheng Zhongyi, Zhu Jun et al.
Scientific Reports 5:None (2015). [PubMed] [Europe PMC]

Integrated proteomic analysis of post-translational modifications by serial enrichment
Mertins Philipp, Qiao Jana W, Patel Jinal et al.
Nature Methods 10:634-637 (2013). [PubMed] [Europe PMC]

Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen L.K., Beli P., Wagner S.A. et al.
Nat. Cell Biol. 14:1089-1098 (2012). [PubMed] [Europe PMC]

204 K Acetylation N6-acetyllysine
Manual assertion inferred from sequence similarity [ECO:0000250]

UniProtKB: Q6IRU5

205 S Phosphorylation Phosphoserine
Peer reviewed articles curated by PhosphoSitePlus®.

Endocytosis of G Protein-Coupled Receptors Is Regulated by Clathrin Light Chain Phosphorylation
Ferreira Filipe, Foley Matthew, Cooke Alex et al.
Current Biology 22:1361-1370 (2012). [PubMed] [Europe PMC]

207 K Ubiquitination Ubiquitination-Lys-Gly
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
  • Curation set 8673 (2009)
  • Curation set 8639 (2009)
207 K Methylation N6-methyllysine
Peer reviewed articles curated by PhosphoSitePlus®.

Quantitative Profiling of the Activity of Protein Lysine Methyltransferase SMYD2 Using SILAC-Based Proteomics
Olsen Jonathan B., Cao Xing-Jun, Han Bomie et al.
Molecular & Cellular Proteomics 15:892-905 (2016). [PubMed] [Europe PMC]

217 S Phosphorylation Phosphoserine
Mass spectrometry data [ECO:0007829]

From CPTAC3 GBM discovery

Proteogenomic and metabolomic characterization of human glioblastoma
Wang Liang-Bo, Karpova Alla, Gritsenko Marina A. et al.
Cancer Cell 39:509-528.e20 (2021). [PubMed] [Europe PMC]

From CPTAC3 HNSCC discovery

Proteogenomic insights into the biology and treatment of HPV-negative head and neck squamous cell carcinoma
Huang Chen, Chen Lijun, Savage Sara R. et al.
Cancer Cell 39:361-379.e16 (2021). [PubMed] [Europe PMC]

From CPTAC3 LUAD discovery

Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
Gillette Michael A., Satpathy Shankha, Cao Song et al.
Cell 182:200-225.e35 (2020). [PubMed] [Europe PMC]

From CPTAC3 CCRCC discovery

Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma
Clark David J., Dhanasekaran Saravana M., Petralia Francesca et al.
Cell 179:964-983.e31 (2019). [PubMed] [Europe PMC]

From TCGA BRCA retrospective

Proteogenomics connects somatic mutations to signalling in breast cancer
Mertins Philipp, NCI CPTAC, Mani D. R. et al.
Nature 534:55-62 (2016). [PubMed] [Europe PMC]

From CPTAC3 LSCC discovery

Unpublished data

Peer reviewed articles curated by PhosphoSitePlus®.

Proteogenomics connects somatic mutations to signalling in breast cancer
Mertins Philipp, NCI CPTAC, Mani D. R. et al.
Nature 534:55-62 (2016). [PubMed] [Europe PMC]

Ischemia in Tumors Induces Early and Sustained Phosphorylation Changes in Stress Kinase Pathways but Does Not Affect Global Protein Levels
Mertins Philipp, Yang Feng, Liu Tao et al.
Molecular & Cellular Proteomics 13:1690-1704 (2014). [PubMed] [Europe PMC]

Phosphosignature Predicts Dasatinib Response in Non-small Cell Lung Cancer
Klammer Martin, Kaminski Marc, Zedler Alexandra et al.
Molecular & Cellular Proteomics 11:651-668 (2012). [PubMed] [Europe PMC]

Manual assertion inferred from sequence similarity [ECO:0000250]

UniProtKB: P09496

221 S Phosphorylation Phosphoserine
Peer reviewed articles curated by PhosphoSitePlus®.

Identification of Missing Proteins in the neXtProt Database and Unregistered Phosphopeptides in the PhosphoSitePlus Database As Part of the Chromosome-Centric Human Proteome Project
Shiromizu Takashi, Adachi Jun, Watanabe Shio et al.
Journal of Proteome Research 12:2414-2421 (2013). [PubMed] [Europe PMC]

223 K Ubiquitination Ubiquitination-Lys-Gly
Peer reviewed articles curated by PhosphoSitePlus®.

UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Akimov Vyacheslav, Barrio-Hernandez Inigo, Hansen Sten V. F. et al.
Nature Structural & Molecular Biology 25:631-640 (2018). [PubMed] [Europe PMC]

Integrated proteomic analysis of post-translational modifications by serial enrichment
Mertins Philipp, Qiao Jana W, Patel Jinal et al.
Nature Methods 10:634-637 (2013). [PubMed] [Europe PMC]

Refined Preparation and Use of Anti-diglycine Remnant (K-ε-GG) Antibody Enables Routine Quantification of 10,000s of Ubiquitination Sites in Single Proteomics Experiments
Udeshi Namrata D., Svinkina Tanya, Mertins Philipp et al.
Molecular & Cellular Proteomics 12:825-831 (2013). [PubMed] [Europe PMC]

223 K Acetylation N6-acetyllysine
Mass spectrometry data [ECO:0007829]

From CPTAC3 LSCC discovery

Unpublished data
225 T Phosphorylation Phosphothreonine
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
  • Curation set 9327 (2010)
  • Curation set 9326 (2010)