Canonical isoform: P84243-1 [UniProt]
Other possible isoforms:
HUGO gene symbols:
Protein name: Histone H3.3
Associated HotPTM clusters:
CPTAC:361.11
,
CPTAC:361.3
,
CPTAC:361.7
PTM sites from various resources including CPTAC, PhosphoSitePlus and UniProtKB.
The TCGA PanCancer Atlas MC3 set is a collection of pancancer somatic mutations calls from all TCGA samples. More information can be found on MC3 project page.
COSMIC (the Catalogue Of Somatic Mutations In Cancer) is collection of somatic mutations in cancer with additional manual curation. More information can be found on COSMIC website.
(Only the canonical isoform P84243-1 is shown)
| Position | Residue | Category | PTM Type | Evidence (Manual/Automatic assertion) |
|---|---|---|---|---|
| 3 | R | Methylation | Asymmetric_dimethylarginine |
Manual assertion based on experiment [ECO:0000269]
Arginine methylation of the histone H3 tail impedes effector binding.
Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive.
PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation.
|
| 4 | T | Phosphorylation | Phosphothreonine |
Peer reviewed articles curated by PhosphoSitePlus®.
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment.
Modifications of human histone H3 variants during mitosis.
|
| 5 | K | Acetylation | N6-acetyllysine |
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
|
| 5 | K | Methylation | N6-methyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling *
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Mass spectrometric characterization of human histone H3: a bird's eye view.
Modifications of human histone H3 variants during mitosis.
|
| 5 | K | Methylation | N6,N6,N6-trimethyllysine |
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Mass spectrometric characterization of human histone H3: a bird's eye view.
Modifications of human histone H3 variants during mitosis.
|
| 5 | K | Methylation | N6,N6-dimethyllysine |
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Mass spectrometric characterization of human histone H3: a bird's eye view.
Modifications of human histone H3 variants during mitosis.
|
| 7 | T | Phosphorylation | Phosphothreonine |
Manual assertion based on experiment [ECO:0000269]
Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone H3K4.
|
| 9 | R | Methylation | Symmetric_dimethylarginine |
Manual assertion inferred from sequence similarity [ECO:0000250]UniProtKB: P84244 |
| 10 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line
Lysine Succinylation Is a Frequently Occurring Modification in Prokaryotes and Eukaryotes and Extensively Overlaps with Acetylation
Integrated proteomic analysis of post-translational modifications by serial enrichment
Regulation of cellular metabolism by protein lysine acetylation.
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Mass spectrometric characterization of human histone H3: a bird's eye view.
Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17.
Modifications of human histone H3 variants during mitosis.
|
| 10 | K | Methylation | N6,N6,N6-trimethyllysine |
Peer reviewed articles curated by PhosphoSitePlus®.
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Mass spectrometric characterization of human histone H3: a bird's eye view.
Modifications of human histone H3 variants during mitosis.
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 10 | K | Methylation | N6,N6-dimethyllysine |
Peer reviewed articles curated by PhosphoSitePlus®.
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Mass spectrometric characterization of human histone H3: a bird's eye view.
Modifications of human histone H3 variants during mitosis.
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 10 | K | Methylation | N6-methyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling *
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Mass spectrometric characterization of human histone H3: a bird's eye view.
Modifications of human histone H3 variants during mitosis.
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 11 | S | Phosphorylation | Phosphoserine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
The Plk1-dependent Phosphoproteome of the Early Mitotic Spindle
System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.
Modifications of human histone H3 variants during mitosis.
Serine 31 phosphorylation of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Regulation of ultraviolet B-induced phosphorylation of histone H3 at serine 10 by Fyn kinase.
Chk1-Dependent S-M Checkpoint Delay in Vertebrate Cells Is Linked to Maintenance of Viable Replication Structures
Coregulator Recruitment and Histone Modifications in Transcriptional Regulation by the Androgen Receptor
Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle.
Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis.
Differential Contribution of Inhibitory Phosphorylation of CDC2 and CDK2 for Unperturbed Cell Cycle Control and DNA Integrity Checkpoints
Loss of p53 has site-specific effects on histone H3 modification, including serine 10 phosphorylation important for maintenance of ploidy.
Shear Stress–Mediated Chromatin Remodeling Provides Molecular Basis for Flow-Dependent Regulation of Gene Expression
A nucleosomal function for IκB kinase-α in NF-κB-dependent gene expression
Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation.
Manual assertion based on experiment [ECO:0000269]
The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment.
Serine 31 phosphorylation of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Modifications of human histone H3 variants during mitosis.
Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase.
Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensation.
Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation.
|
| 12 | T | Phosphorylation | Phosphothreonine |
Manual assertion based on experiment [ECO:0000269]
Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation.
Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase.
|
| 15 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line
Lysine Succinylation Is a Frequently Occurring Modification in Prokaryotes and Eukaryotes and Extensively Overlaps with Acetylation
Integrated proteomic analysis of post-translational modifications by serial enrichment
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Regulation of cellular metabolism by protein lysine acetylation.
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17.
Modifications of human histone H3 variants during mitosis.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 15 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Mass spectrometry data [ECO:0007829]From CPTAC3 LSCC discovery Unpublished data |
| 18 | R | Methylation | Asymmetric_dimethylarginine |
Manual assertion based on experiment [ECO:0000269]
Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17.
Human PAD4 regulates histone arginine methylation levels via demethylimination.
Ligand-dependent activation of the farnesoid X-receptor directs arginine methylation of histone H3 by CARM1.
|
| 19 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Mass spectrometry data [ECO:0007829]From CPTAC3 LSCC discovery Unpublished data |
| 19 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Lysine Succinylation Is a Frequently Occurring Modification in Prokaryotes and Eukaryotes and Extensively Overlaps with Acetylation
Integrated proteomic analysis of post-translational modifications by serial enrichment
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
|
| 19 | K | Methylation | N6-methyllysine |
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
|
| 24 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Mass spectrometry data [ECO:0007829]From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Multiomic Analysis of the UV-Induced DNA Damage Response
Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Systematic and Quantitative Assessment of the Ubiquitin-Modified Proteome
A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles
|
| 24 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC3 GBM discovery
Proteogenomic and metabolomic characterization of human glioblastoma
From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 UCEC discovery
Proteogenomic Characterization of Endometrial Carcinoma
From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line
Lysine Succinylation Is a Frequently Occurring Modification in Prokaryotes and Eukaryotes and Extensively Overlaps with Acetylation
Integrated proteomic analysis of post-translational modifications by serial enrichment
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 24 | K | Methylation | N6-methyllysine |
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
|
| 28 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC3 GBM discovery
Proteogenomic and metabolomic characterization of human glioblastoma
From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 UCEC discovery
Proteogenomic Characterization of Endometrial Carcinoma
From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Manual assertion inferred from algorithms based on publications or databases entries, e.g., large-scale proteomics data and 3D structure [ECO:0007744]
Lysine acetylation targets protein complexes and co-regulates major cellular functions.
|
| 28 | K | Methylation | N6,N6,N6-trimethyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome
Large-scale global identification of protein lysine methylation in vivo
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Modifications of human histone H3 variants during mitosis.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 28 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Mass spectrometry data [ECO:0007829]From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Systematic and Quantitative Assessment of the Ubiquitin-Modified Proteome
|
| 28 | K | Methylation | N6-methyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Quantitative Profiling of the Activity of Protein Lysine Methyltransferase SMYD2 Using SILAC-Based Proteomics
A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling *
Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
Large-scale global identification of protein lysine methylation in vivo
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Modifications of human histone H3 variants during mitosis.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 28 | K | Methylation | N6,N6-dimethyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Modifications of human histone H3 variants during mitosis.
Human spleen histone H3. Isolation and amino acid sequence.
Manual assertion inferred from algorithms based on publications or databases entries, e.g., large-scale proteomics data and 3D structure [ECO:0007744]
Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
|
| 29 | S | Phosphorylation | Phosphoserine |
Mass spectrometry data [ECO:0007829]From CPTAC3 GBM discovery
Proteogenomic and metabolomic characterization of human glioblastoma
From CPTAC3 HNSCC discovery
Proteogenomic insights into the biology and treatment of HPV-negative head and neck squamous cell carcinoma
From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC2 OV prospective
Proteogenomic Characterization of Ovarian HGSC Implicates Mitotic Kinases, Replication Stress in Observed Chromosomal Instability
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 UCEC discovery
Proteogenomic Characterization of Endometrial Carcinoma
From CPTAC2 CRC prospective
Proteogenomic Analysis of Human Colon Cancer Reveals New Therapeutic Opportunities
From CPTAC3 CCRCC discovery
Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma
From TCGA BRCA retrospective
Proteogenomics connects somatic mutations to signalling in breast cancer
From CPTAC3 LSCC discovery Unpublished data Peer reviewed articles curated by PhosphoSitePlus®.
Proteogenomics connects somatic mutations to signalling in breast cancer
Ultradeep Human Phosphoproteome Reveals a Distinct Regulatory Nature of Tyr and Ser/Thr-Based Signaling
Ischemia in Tumors Induces Early and Sustained Phosphorylation Changes in Stress Kinase Pathways but Does Not Affect Global Protein Levels
Identification of Missing Proteins in the neXtProt Database and Unregistered Phosphopeptides in the PhosphoSitePlus Database As Part of the Chromosome-Centric Human Proteome Project
The Plk1-dependent Phosphoproteome of the Early Mitotic Spindle
Quantitative Phosphoproteomics Identifies Substrates and Functional Modules of Aurora and Polo-Like Kinase Activities in Mitotic Cells
Quantitative Site-specific Phosphorylation Dynamics of Human Protein Kinases during Mitotic Progression
Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.
Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.
Modifications of human histone H3 variants during mitosis.
Serine 31 phosphorylation of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis.
Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation.
Manual assertion based on experiment [ECO:0000269]
The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment.
Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha.
Serine 31 phosphorylation of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Modifications of human histone H3 variants during mitosis.
Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensation.
Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation.
|
| 32 | S | Phosphorylation | Phosphoserine |
Mass spectrometry data [ECO:0007829]From CPTAC3 HNSCC discovery
Proteogenomic insights into the biology and treatment of HPV-negative head and neck squamous cell carcinoma
From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC2 OV prospective
Proteogenomic Characterization of Ovarian HGSC Implicates Mitotic Kinases, Replication Stress in Observed Chromosomal Instability
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 UCEC discovery
Proteogenomic Characterization of Endometrial Carcinoma
From CPTAC2 CRC prospective
Proteogenomic Analysis of Human Colon Cancer Reveals New Therapeutic Opportunities
From CPTAC3 CCRCC discovery
Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma
From TCGA BRCA retrospective
Proteogenomics connects somatic mutations to signalling in breast cancer
From CPTAC3 LSCC discovery Unpublished data Peer reviewed articles curated by PhosphoSitePlus®.
Proteogenomics connects somatic mutations to signalling in breast cancer
CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells
Ultradeep Human Phosphoproteome Reveals a Distinct Regulatory Nature of Tyr and Ser/Thr-Based Signaling
Quantitative Phosphoproteomics Identifies Substrates and Functional Modules of Aurora and Polo-Like Kinase Activities in Mitotic Cells
Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells
Modifications of human histone H3 variants during mitosis.
Serine 31 phosphorylation of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Manual assertion based on experiment [ECO:0000269]
Serine 31 phosphorylation of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Modifications of human histone H3 variants during mitosis.
|
| 33 | T | Phosphorylation | Phosphothreonine |
Mass spectrometry data [ECO:0007829]From CPTAC3 UCEC discovery
Proteogenomic Characterization of Endometrial Carcinoma
From CPTAC2 CRC prospective
Proteogenomic Analysis of Human Colon Cancer Reveals New Therapeutic Opportunities
|
| 37 | K | Methylation | N6-methyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Quantitative Profiling of the Activity of Protein Lysine Methyltransferase SMYD2 Using SILAC-Based Proteomics
Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells
A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling *
Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome
Large-scale global identification of protein lysine methylation in vivo
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Modifications of human histone H3 variants during mitosis.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 37 | K | Methylation | N6,N6,N6-trimethyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Modifications of human histone H3 variants during mitosis.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 37 | K | Methylation | N6,N6-dimethyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
Modifications of human histone H3 variants during mitosis.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Modifications of human histone H3 variants during mitosis.
Human spleen histone H3. Isolation and amino acid sequence.
|
| 37 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC3 GBM discovery
Proteogenomic and metabolomic characterization of human glioblastoma
From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 UCEC discovery
Proteogenomic Characterization of Endometrial Carcinoma
From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Manual assertion based on experiment [ECO:0000269]
Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification.
Organismal differences in post-translational modifications in histones H3 and H4.
|
| 37 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Mass spectrometry data [ECO:0007829]From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Systematic and Quantitative Assessment of the Ubiquitin-Modified Proteome
|
| 38 | K | Methylation | N6-methyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Quantitative Profiling of the Activity of Protein Lysine Methyltransferase SMYD2 Using SILAC-Based Proteomics
Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells
Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome
Manual assertion inferred from sequence similarity [ECO:0000250]UniProtKB: P68431 |
| 38 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC3 UCEC discovery
Proteogenomic Characterization of Endometrial Carcinoma
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
|
| 38 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
|
| 38 | K | Methylation | N6,N6-dimethyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
|
| 38 | K | Methylation | N6,N6,N6-trimethyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
H3F3 mutation status of giant cell tumors of the bone, chondroblastomas and their mimics: a combined high resolution melting and pyrosequencing approach
|
| 41 | R | Methylation | Omega-N-methylarginine |
Peer reviewed articles curated by PhosphoSitePlus®.
Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells
Proteomic Analysis of Arginine Methylation Sites in Human Cells Reveals Dynamic Regulation During Transcriptional Arrest
Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome
|
| 42 | Y | Phosphorylation | Phosphotyrosine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Identification of Missing Proteins in the neXtProt Database and Unregistered Phosphopeptides in the PhosphoSitePlus Database As Part of the Chromosome-Centric Human Proteome Project
Survey of Tyrosine Kinase Signaling Reveals ROS Kinase Fusions in Human Cholangiocarcinoma
Manual assertion based on experiment [ECO:0000269]
JAK2 phosphorylates histone H3Y41 and excludes HP1alpha from chromatin.
|
| 46 | T | Phosphorylation | Phosphothreonine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics
Ischemia in Tumors Induces Early and Sustained Phosphorylation Changes in Stress Kinase Pathways but Does Not Affect Global Protein Levels
Toward a comprehensive characterization of a human cancer cell phosphoproteome.
The Plk1-dependent Phosphoproteome of the Early Mitotic Spindle
Specificity Determinants of Substrate Recognition by the Protein Kinase DYRK1A
|
| 57 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 LSCC discovery Unpublished data Peer reviewed articles curated by PhosphoSitePlus®.
Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
|
| 57 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Mass spectrometry data [ECO:0007829]From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Site-specific identification and quantitation of endogenous SUMO modifications under native conditions
Multiomic Analysis of the UV-Induced DNA Damage Response
Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line
Integrated proteomic analysis of post-translational modifications by serial enrichment
Refined Preparation and Use of Anti-diglycine Remnant (K-ε-GG) Antibody Enables Routine Quantification of 10,000s of Ubiquitination Sites in Single Proteomics Experiments
Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Systematic and Quantitative Assessment of the Ubiquitin-Modified Proteome
A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles
A Data Set of Human Endogenous Protein Ubiquitination Sites
Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling
|
| 57 | K | Methylation | N6,N6,N6-trimethyllysine |
Manual assertion based on experiment [ECO:0000269]
Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA.
Organismal differences in post-translational modifications in histones H3 and H4.
|
| 57 | K | Methylation | N6-methyllysine |
Manual assertion based on experiment [ECO:0000269]
Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA.
Organismal differences in post-translational modifications in histones H3 and H4.
|
| 58 | S | Phosphorylation | Phosphoserine |
Mass spectrometry data [ECO:0007829]From CPTAC3 CCRCC discovery
Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Proteogenomics connects somatic mutations to signalling in breast cancer
A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells*
Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors
Identification of Missing Proteins in the neXtProt Database and Unregistered Phosphopeptides in the PhosphoSitePlus Database As Part of the Chromosome-Centric Human Proteome Project
Toward a comprehensive characterization of a human cancer cell phosphoproteome.
The Plk1-dependent Phosphoproteome of the Early Mitotic Spindle
Phosphoproteome Analysis of Functional Mitochondria Isolated from Resting Human Muscle Reveals Extensive Phosphorylation of Inner Membrane Protein Complexes and Enzymes
Quantitative Phosphoproteomics Identifies Substrates and Functional Modules of Aurora and Polo-Like Kinase Activities in Mitotic Cells
Global phosphorylation analysis of -arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR)
Manual assertion based on experiment [ECO:0000269]
Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.
|
| 59 | T | Phosphorylation | Phosphothreonine |
Peer reviewed articles curated by PhosphoSitePlus®.
Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors
Toward a comprehensive characterization of a human cancer cell phosphoproteome.
The Plk1-dependent Phosphoproteome of the Early Mitotic Spindle
Phosphoproteome Analysis of Functional Mitochondria Isolated from Resting Human Muscle Reveals Extensive Phosphorylation of Inner Membrane Protein Complexes and Enzymes
Quantitative Phosphoproteomics Identifies Substrates and Functional Modules of Aurora and Polo-Like Kinase Activities in Mitotic Cells
|
| 65 | K | Methylation | N6-methyllysine |
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
|
| 80 | K | Methylation | N6-methyllysine |
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks.
|
| 80 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Mass spectrometry data [ECO:0007829]From CPTAC3 LSCC discovery Unpublished data Peer reviewed articles curated by PhosphoSitePlus®.
UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites
Site-specific identification and quantitation of endogenous SUMO modifications under native conditions
Multiomic Analysis of the UV-Induced DNA Damage Response
Integrated proteomic analysis of post-translational modifications by serial enrichment
Refined Preparation and Use of Anti-diglycine Remnant (K-ε-GG) Antibody Enables Routine Quantification of 10,000s of Ubiquitination Sites in Single Proteomics Experiments
Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Systematic and Quantitative Assessment of the Ubiquitin-Modified Proteome
A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles
Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling
|
| 80 | K | Methylation | N6,N6,N6-trimethyllysine |
Manual assertion inferred from sequence similarity [ECO:0000250]UniProtKB: P84244 |
| 80 | K | Methylation | N6,N6-dimethyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.
Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome
A General Molecular Affinity Strategy for Global Detection and Proteomic Analysis of Lysine Methylation
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks.
|
| 80 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC2 BRCA prospective
Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy
From CPTAC3 LUAD discovery
Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma
From CPTAC3 LSCC discovery Unpublished data Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Regulation of cellular metabolism by protein lysine acetylation.
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
|
| 81 | T | Phosphorylation | Phosphothreonine |
Mass spectrometry data [ECO:0007829]From CPTAC3 CCRCC discovery
Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Proteogenomics connects somatic mutations to signalling in breast cancer
A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells*
Ischemia in Tumors Induces Early and Sustained Phosphorylation Changes in Stress Kinase Pathways but Does Not Affect Global Protein Levels
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
Quantification of Pancreatic Cancer Proteome and Phosphorylome: Indicates Molecular Events Likely Contributing to Cancer and Activity of Drug Targets
Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors
Identification of Missing Proteins in the neXtProt Database and Unregistered Phosphopeptides in the PhosphoSitePlus Database As Part of the Chromosome-Centric Human Proteome Project
Toward a comprehensive characterization of a human cancer cell phosphoproteome.
Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues.
Manual assertion based on experiment [ECO:0000269]
Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.
|
| 87 | S | Phosphorylation | Phosphoserine |
Mass spectrometry data [ECO:0007829]From CPTAC3 GBM discovery
Proteogenomic and metabolomic characterization of human glioblastoma
From CPTAC3 HNSCC discovery
Proteogenomic insights into the biology and treatment of HPV-negative head and neck squamous cell carcinoma
From CPTAC3 CCRCC discovery
Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma
Peer reviewed articles curated by PhosphoSitePlus®.
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
Manual assertion inferred from algorithms based on publications or databases entries, e.g., large-scale proteomics data and 3D structure [ECO:0007744]
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
|
| 108 | T | Phosphorylation | Phosphothreonine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Peer reviewed articles curated by PhosphoSitePlus®.
Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
Manual assertion inferred from sequence similarity [ECO:0000250]UniProtKB: Q71DI3 |
| 116 | K | Ubiquitination | Ubiquitination-Lys-Gly |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
|
| 116 | K | Acetylation | N6-acetyllysine |
Cell Signaling Technology® internal validation data by PhosphoSitePlus®.
Manual assertion based on experiment [ECO:0000269]
Acetylation of histone H3 at the nucleosome dyad alters DNA-histone binding.
|
| 123 | K | Acetylation | N6-acetyllysine |
Mass spectrometry data [ECO:0007829]From CPTAC3 LSCC discovery Unpublished data Peer reviewed articles curated by PhosphoSitePlus®.
Suberoylanilide Hydroxamic Acid Treatment Reveals Crosstalks among Proteome, Ubiquitylome and Acetylome in Non-Small Cell Lung Cancer A549 Cell Line
Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Manual assertion based on experiment [ECO:0000269]
Regulation of transcription through acetylation of H3K122 on the lateral surface of the histone octamer.
Acetylation of histone H3 at the nucleosome dyad alters DNA-histone binding.
|
| 123 | K | Methylation | N6-methyllysine |
Manual assertion based on experiment [ECO:0000269]
Organismal differences in post-translational modifications in histones H3 and H4.
Expression patterns and post-translational modifications associated with mammalian histone H3 variants.
|